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KMID : 1161520160200020063
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Animal Cells and Systems
2016 Volume.20 No. 2 p.63 ~ p.69
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miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat
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Ji Zhibin
Wang Guizhi
Hou Lei
Liu Zhaohua
Wang Jianmin
Chao Tianle
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Abstract
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MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.
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KEYWORD
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MicroRNA, mammary epithelial cell, flow cytometry, hoechst33342/PI
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